Composite

Part:BBa_K2203003:Design

Designed by: Malek Kabani   Group: iGEM17_EPFL   (2017-10-22)


HepHCV_ToeholdA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Touchdown PCR to connect the toehold to the lacZalpha fragment


Source

The toehold sequence was generated by the software proposed by 2017.igem.org/Team:EPFL and the lacZalpha coding sequence extracted from BBa_I732006. Subsequently the two fragments were connected by PCR.


References